反义寡核苷酸对多药抗性基因表达影响的研究

The Study of Antisense Oligonucleotides′ Effect on Mdrl Gene Expression

多药抗性现象是肿瘤有效化疗的主要障碍 ,其中 mdr1基因过量表达产生膜蛋白 Pg P是最关键的一种。利用该多药抗性表型标志建立了 ELISA法定量检测 mdr1的表达水平 ,并用反义寡核苷酸对基因的表达进行调控。结果表明天然寡核苷酸在 Lipofectin的介导下能一定程度地抑制 Pg

Multidrug resistance is the main obstacle in tumor chemotherapy.Among its complex contributing factors,overexpression of PgP membrane protein is a key one. According to this cell mark,we set up ELISA method to detect mdr1 gene level and use antisense oligonucleotides(ASON) to regulate mdr1 expression.The results at present show that with the help of lipofectin,natural ASON can inhibit the expression of mdr1 to a certain extent.