摘要
用自制的亲和层析柱纯化来自尖吻蝮蛇毒的降纤酶 ,可得到符合部颁质量标准的产品。降纤酶的 SDS- PAGE纯度为单一酶带 ,HPLC纯度为单峰 ,酶比活为 2 886U/ mg,与离子交换层析和凝胶过滤法比较 。
Defibrase had been purified from the venom of Agkistrondon acutus by affinity chromatography. The quality of defibrase fits the standards made by the Ministry of Healty. The purified defibrase is homogenous in SDS PAGE electrophoresis, and a single peak in HPLC. The specific activity is 2886U/mg. Comparing with ion exchange chromatography and gel filtration, this method fits in with the need of defibrase manufacture.
出处
《药物生物技术》
CAS
CSCD
1998年第4期238-240,共3页
Pharmaceutical Biotechnology
关键词
亲和层析
降纤酶
电泳
高效液相色谱
Affinity chromatography, Agkistrondon acutus venom, Electrophoresis, HPLC
