萝卜种子超干处理与种子活力及脂质过氧化关系

Radish Seeds with the Ultradry Seed Vigor and Lipid Peroxidation in Relations

将不同含水量的萝卜种子通过直接吸胀萌发、回水萌发、老化回水等处理,测定种子发芽率、活力指数、发芽指数,种子浸出液电导率、丙二醛、过氧化氢酶和过氧化物酶含量等,研究超干处理与种子活力及脂质过氧化的关系。结果表明:随种子含水量降低,种子直接吸胀萌发,发芽指数和活力指数均不同程度下降;萌发前回水处理,发芽指数和活力指数与自然含水量的基本一致;老化回水处理,自然含水量种子活力大幅度下降,超干种子仍保持较高水平;超干种子活力显著高于对照,脂质过氧化程度低于对照,耐贮性显著提高。萝卜种子超干贮藏最适含水量在3%左右。

The trial of radish seeds of material evaluated the relationship of the dry seed vigor and lipid oxidation at room temperature silica drying their moisture content（MC） from 7. 35% to 4. 84%, 3. 57% ,2. 49% and 1.86% ,by measuring levels of various seed vigor indicators, germination rate, vigor index, germination index, lipid peroxidation indicators conductivity, catalase content, peroxidase content and MDA. The results showed that Ultradry seeds（MC= 4. 84% 1. 86%） direct imbibition germination index and vitality index had declined;imbibed before giving back its vitality basic treatment and control（MC= 7.35 %） were unanimous. It was to ensure the vitality of seeds key factor that the appropriate treatment could reduce the imbibition process of the injury. After aging treatment, control of seed vigor bad decreased considerably and uhradry seed vigor remained unchanged. Uhradry could significantly improved the seed agingresistance capability. In addition, the aging water treatment to uhradry seeds of the external fluid conductivity was significantly lower than the control seeds;Lipid peroxidation product malondialdehyde content was significantly higher than the control. Note uhradry aging seed membrane to maintain the physiological function of a more comprehensive and enzyme system remained intact, It collaborated with non-enzymatic reactions common scavenging free radicals such as poison substances, or to prevent a weakening of lipid peroxidation happening, make ultra-dry seed showed good storability. Therefore, the use of ultradry turnip germplasm storage technology was feasible.