低温预处理对刺梨花药培养小孢子发育的影响

The Effect of Cold Pretreatment on the Development of Microspores During in Vitro Culture of Rose roxburghii Trattcv.Anther

为明确低温预处理对刺梨花药离体培养过程中小孢子发育及愈伤组织诱导率的影响,进行低温预处理试验,结果表明:离体培养条件下,刺梨雄核存在3条发育途径,即A-V途径、A-G途径和B途径,以A途径为主;随着培养时间的增加,小孢子逐渐退化,低温预处理3d可在一定程度上延缓刺梨小孢子退化,提早雄核发育,并增加参与雄核发育小孢子数量,提高花药愈伤组织诱导率。

A cold pretreatment experiment was carried out on Rose roxburghii Trattcv.anther culture to study the effect of cold pretreatment on the development of microspores during Rose roxburghii Trattcv.anther culture in vitro.The results indicated that：three division ways existed in androgenesis of Rose roxburghii Trattcv.anther culture,namely pathway A-V,pathway A-G and pathway B.And pathway A was the predominent way.Along with prologation of the culthure time,degeneration happened with some microspores.Cold pretreatment for 3 d could delay the degeneration,and enhance the ratio of microspores participated in androgenesis.Cold pretreatment for 3 d increased callus induction frequency of Rose roxburghii Trattcv.anther.