摘要
采用PCR-SSCP技术,对27例原发性非小细胞肺癌(NSCLC)的癌组织标本进行了P16基因外显子2的缺失和突变的检测。结果发现6例基因缺失和3例基因突变,且P16基因的变异多发生在NSCLC的晚期。结论P16基因的变异在NSCLC的形成和发展中起重要作用。
In order to observe the deletion and mutation of P16 gene in nonsmall cell lung cancer,we examined the alteration in exon 2 of P16 gene among 27 patients with NSCLC by PCR and PCRSSCP.The results demonstrated that there were 6 cases of deletion and 3 cases of mutation in these patients.It is suggested that the alteration of p16 gene is important in the pathogenesis of NSCLC.