白细胞介素-1家族在小鼠阿霉素心脏毒性模型中的表达

The expression of interleukin-1 family in mice with adriamycin-induced cardiotoxicity

目的:探讨白细胞介素-1家族成员白细胞介素-1β(IL-1β),白细胞介素-1受体拮抗剂(IL-1Ra),白细胞介素-1(IL-1)受体I(IL-1RI)在小鼠急性阿霉素心脏毒性模型中的表达及其与心脏毒性的可能内在联系。方法:通过一次性腹腔注射阿霉素(15mg/kg),建立小鼠急性阿霉素心脏毒性模型。在给药前及给药后第3、7、14天分别取小鼠血清行ELISA法检测血清中IL-1β、IL-1Ra表达变化,并取小鼠心肌标本通过免疫组化、western blot法检测小鼠心肌组织中IL-1RI表达变化。同时,通过心肌组织病理学观察评价小鼠心肌损伤程度。结果:小鼠血清中IL-1β、IL-1Ra及心肌组织中IL-1RI均于阿霉素注射后表达升高,并于第7天达到最高。而心肌组织病理学观察显示小鼠在给药后第7天损伤明显。结论:IL-1家族在小鼠阿霉素心脏毒性模型中表达升高,提示其可能参与了阿霉素所致的小鼠心脏毒性损伤过程。

Objective：To study the expression of interleukin-1β（IL-1β）, interleukin-1 receptor antagonist （IL-1Ra） and IL-1 receptor I （IL-1RI） in mice with acute adriamycin（ADR）-induced cardiotoxicity and try to find their potential correlations. Methods：Mouse models of acute cardiotoxicity was established by intraperitoneal injection of ADR （15 mg/kg）. The expression of IL-1β and IL-1Ra in serum was detected by enzyme-linked immunosorbent assay （ELISA）, while the expression of IL-1RI in cardiac tissues was detected by immunohistochemical staining and Western blot approaches. Cardiac tissue sections were used to evaluate the cardiac damage. Results：After ADR treatment, IL-1βand IL-1Ra concentrations in serum were highly induced and reached the maximum on day 7. IL-1RI protein in cardiac tissues was also expressed and reached the highest on day 7. Histopathological analysis of cardiac tissues showed obvious cardiac damage after 7 days of ADR treatment. Conclusion.. IL-1 family are highly induced and may be involved in the cardiotoxicity after ADR treatment.