胃癌病人手术前后血清细胞因子分析

ANALYSIS OF SERUM CYTOKINES LEVELS IN GASTRIC CANCER PATIENTS BEFORE AND AFTER SURGICAL OPERATION

采用放射免疫方法,对30例健康对照人员及20例晚期胃癌、11例直肠癌病人,分别测定了手术前,手术中、手术后一天以及手术后七天患者血清的肿瘤坏死因子(TNF)、白细胞介素-2(IL-2)和上皮细胞生长因子(EGF)。结果分别为TNF在手术前、手术中及手术后一天同对照组没有显著性差别(p>0.05),而手术后七天升高,与对照组和手术前组比较均有显著性差别;IL-2表现为手术前、手术后一天、七天均降低,同对照组比较有显著性差异,并且术前与术后比较也有显著性差别,而手术组呈现升高现象,与对照组没有差别;EGF表现为术前、术中、术后均高于对照组(p<0.01)。结果表明,恶性肿瘤病人,细胞免疫功能低下,手术后有所改善。因此,对手术后病人进行动态检测细胞因子,对恶性肿瘤的疗效及预后判断均有指导意义。

RIA was used to detect the sera levels of tumor necrosis factor (TNF), interleukin-2 (IL-2) and epidermal growth factor (EGF) in 20 cases with gastric cancer and 11 cases with rectal cancer as well as 30 controls. The patients' sera were taken before operation, during operation, one day after operation and seven days after operation respectively. The results showed that TNF levels before operation, during operation and at one day after operation had no significant change (p>0.05) compared with those of the controls but at seven days after operation were significantly higher. The levels of IL-2 before operation, one day after operation and at seven days after operation were significantly lower than those of the control group. IL-2 levels also changed significantly after operation; IL-2 levels also raised during operation but had no significance compared with the control ones EGF levels were always significantly higher in the cancer patients (p <0.01).The results showed that patients with malignant tumor had lowered immune function but it was improved after operation. It was thought that continual monitoring of cytokines levels in patients with malignant tumor after operation might help to judge the prognosis.