低氧训练对大鼠心肌组织微血管生成的影响

Effect of Hypoxic Training to Promote Myocardial Angiogenesis of Rats

目的研究不同的低氧训练模式大鼠心肌组织微血管的生成情况和变化规律,为低氧训练实践提供试验依据。方法将健康雄性SD大鼠60只,按体重随机分为6组,每组10只。运动组采用10周递增负荷跑台运动训练,每周训练6天,运动量由第1周的速度为15 m/min、持续时间为25 min递增至第10周速度为28 m/min、持续时间为50 min,低氧训练组每周二、四、六在相当于海拔1 500 m的低氧环境中训练,一、三、五在常氧下训练。并且在低氧环境中居住,低氧环境由第1周相当于海拔1 800 m递增至第10周相当于海拔3 600 m。应用免疫组织化学、显微图象对CD34的阳性表达进行定性和定量分析。结果CD34可较好标记心肌组织微血管,运动组与低氧训练组有丰富的微血管新生。结论单纯低氧不能显著增加心肌组织的血管生成,运动能使心肌组织血管产生适应性变化,当低氧与运动两种因素同时介入,血管生成丰富。

Objective：In order to provide experimental basis for hypoxie training practice, through studying the effects and rules of rat myocardial angiogenesis under different model of hypoxic training . Methods： 60 male SD rats were randomly divided into six groups, 10 rats each. 10 weeks progressive treadmill exercise program were designed with intensity starts from 15m/min for 25min at the first week to 28 m/min for 50 min in the last week and hypoxia degree from 1800m at the first week to 3 600 m in the last week. And the hypoxic training groups were trained in the hypoxia cabin,oxygen concentration equal to 1 500 m height every week2.4.6, every week1.3.5were trained in normal oxygen concentration environment, uses the immunity histochemistry and the micro image analysis to the positive expression of CD34. Results： CD34 may be a good marker of myocardial microvascular, including hypoxia training group a large number of CD34 protein expression. Conclusion： Hypoxia alone can not be increased in myocardial angiogenesis, exercise can produce adaptive changes, but hypoxic training can increased angiogenesis singnificantly.