核黄素的循环伏安特性及生物学功能

Cyclic Voltametric Properties of Riboflavin and Its Bioactivities

利用循环伏安法(CV)研究核黄素(RF)在不同酸度、光照后的循环伏安特性和其氧化还原的反应机理,并探讨还原型谷胱甘肽(GSH)与RF之间的相互作用和GSH对呼吸链中RF骨架的氧化还原作用的影响.研究结果表明:在RF的异咯嗪骨架中,不饱和氮原子在强酸性溶液中通过质子化发生两步单电子传递的氧化还原作用;RF在光照后降解生成光色素,也发生两步单电子传递的氧化还原作用;相对低浓度的GSH对RF异咯嗪骨架的两电子传递作用具有激励作用,但高浓度的GSH对RF的氧化还原作用具有抑制作用.

Changes of cyclic voltametric properties of riboflavin（RF） has been studied when the solutions exposured to the light and added reduced glutathione（GSH） with different adicities. RF showed two step redox reactions at the nitrogen double bond through protinization in the highly acidic solution. Two step redox reaction of RF solution was also observed after lignth exposure of the solution. In the mixture solutions of RF and GSH, relatively lower concentration of GSH might enhance two step redox reactions at nitrogen double bonds of RF and relatively higher concentration 05 GSH inhibit redox reactions at the both site of nitrogen double bonds of RF. The redox reaction mechanism and related bioacitivities has also been investigated.