纳豆多糖对环磷酰胺所致免疫低下小鼠的肝肾抗氧化能力的影响

Effects of Natto Polysaccharide on The Antioxidant Capacity of Immuno - suppressive Mice Induced by Cyclophosphamide

为了阐明纳豆多糖对小鼠肝和肾抗氧化能力的影响和最适作用浓度。用昆明种小鼠以环磷酰胺(80mg/kg)造成免疫低下动物模型,然后以纳豆多糖(200,500mg/kg)灌胃2周。试验中进行了一般症状的观察及体重变化的测定,测定了脏器指数,进行了肝肾的抗氧化特性的检查。试验结果表明,与对照组相比纳豆多糖高剂量组(500mg/kg)、低剂量组(200mg/kg)小鼠肝肾中的丙二醛(MDA)含量明显降低(P<0.05),且纳豆高剂量组和低剂量组比对照组肝、肾组织中的超氧化物歧化酶(SOD)活力明显升高,纳豆多糖对小鼠肝肾产生抗氧化效果的合适剂量为200mg/kg。

To investigate the antioxidant effects of the Natto polysaccharide on the liver and kidney of immuno-suppressive mice induced by cyclophosphamide and find the optimum concentration. A total of 40 Kunming mice weighing （20±2） g were randomly divided into four groups, namely, normal control group, cyclophosphamide treatment model group, high-dose Natto treatment group （500 mg/kg） and low-dose Natto treatment group （200 mg/kg）. All mice in the groups except normal control group were administrated with cyclophosphamide at the dosage of 80 mg/kg, and then the two Natto treatment groups were given Natto polysaccharide at the dosage of 500 and 200 mg/kg by garage, respectively for two weeks. The results showed that the Malondialdehyde （MDA） contents of liver and kidney in Natto treatment groups were both significantly decreased （P 〈 0.05） compared with that of cyclophosphamide treatment model group, but the superoxide dismutase （SOD） contents of liver and kidney in Natto treatment groups were greatly increased compared with that of cyclophosphamide treatment model group. Natto polysaccharide has antioxidation function and the optimum dose is 200 mg/kg in mice.