利用STR位点进行牛亲子鉴定的研究

Parentage Identification of Cattle by Short Tandem Repeat Loci

为利用STR分型技术进行牛亲子鉴定,运用PCR技术用TGLA227、BM2113、TGLA53、ETH10、SPS115、TGLA126、TGLA122、INRA23、ETH3、ETH225、BM1824共11个STR(短串联重复序列)基因座对争议小牛、1号嫌疑母牛、2号嫌疑母牛的DNA进行扩增,扩增产物经遗传分析仪检测,检测结果用GeneMapper片段分析软件分析。鉴定结果可以排除1号母牛与争议小牛间具有亲子关系,不排除争议小牛与2号母牛间具有亲子关系。结果表明:STR基因分型技术能够准确、快速地进行牛亲子鉴定,该研究所用的STR位点具有较高的鉴别效率。

A total of ll short tandem repeat （STR） loci including TGLA227,BM2113,TGLA53,ETHlO,SPS115,TGLA126, TGLA 122 ,INRA 23 ,ETH3 ,ETH225 and BM1824 on DNA of three cattle, namely, calf, the first cow and the second cow were amplified by PC R method, the amplification products were separated and analyzed in a genetic analyzer （AB 3130xl）. The paternity probability between the calf and the first cow was ruled out, but that was not ruled out between the calf and the second cow. The results showed that STR loci analysis was a validation method for identification parentage in cattle. Most of the STR loci using in the present study has high performance.