纳米银粒子在微乳液中的制备及其抗菌性能

Preparation of Silver Nanoparticles in Microemulsion System and Its Antibacterial Property

在以二丁酸二异辛酯磺酸钠为表面活性剂、异辛烷为油相形成的W/O型微乳体系中,以AgNO3为银源、抗坏血酸为还原剂,讨论了AgNO3浓度、抗坏血酸浓度以及水核半径(W)对制备纳米银粒子的影响,利用紫外-可见光谱分析(UV-Vis)、透射电镜(TEM)和纳米粒度仪对制得的纳米银进行表征。结果表明,在AgNO3浓度为0.2mol/L,抗坏血酸浓度为0.2mol/L,W值为10的条件下,得到大小为10nm左右,单分散性好的均匀球状纳米银溶胶。抗菌性能测试表明,当纳米银粒子质量浓度为10μg/mL时,对大肠杆菌的杀菌率达98%以上。

Silver nanoparticles were synthesized in water-in-oil microemulsion using silver nitrate solubilized in the water core of a microemulsion as source of silver ions, ascorbic acid solubilized in the water core of another one as reducing agent, isooctane as the continuous phase, and sodium bis （2-ethylhexyl） sulfosuccinate as the surfactant. The influence of AgNOa concentration, ascorbic acid concentration and the molar ratios of water to surfactant （W） in microemulsionon on the silver nanoparticles have been investigated by TEM, UV-Vis spectra and Zetasizer Nano. As a result, the silver nanoparticles possessed good dispersivity, and their diameters were about 10 nm when the concentration of AgNO3 was 0.2 tool / L, the concentration of ascorbic acid was 0.2 mol / L and the water core radius （W） was 10. The antibacterial property of the silver nanoparticles indicated the E.coli was completely killed when the concentration of the silver nanoparticles was 10 μg/mL.