HSV及其实验室检测研究进展(综述)

Study on HSV and Its Laboratory Assay

单纯疱疹病毒(HSV)是一类严重危害人类健康、引起人类多种疾病的常见病原体,尚缺乏简便有效、临床实用的实验室检测手段。目前实验室检测方法主要有:病毒分离培养、免疫荧光检测(IFA法)、分子生物学检测(PCR法)、免疫印迹检测(WB法)以及抗体检测(ELISA法),但仍以从水疱或体液中分离培养出HSV病毒作为"金标准"。

Herpes simplex virus （HSV） is a common serious pathogen which is harmful to human health and cause many diseases for human.It still lacks of simple and effective means of clinical laboratory testing about HSV.The current laboratory assay methods are mainly：the virus isolation and culture,immunofluorescence assay （IFA）,molecular biology （PCR）,Western blotting （WB） and antibodies assay （ELISA）.But the virus isolation and culture of HSV from blisters or body fluids is still a ＂gold standard＂ to Laboratory assay HSV.