神经生长因子对人视网膜血管内皮细胞的影响

Effect of NGF on Cultured Human Retinal Capillary Endothelial Cells (HRCEC)

【目的】观察神经生长因子(NGF)及TrKa阻断剂K252a对体外培养的人视网膜血管内皮细胞(HRCEC)增殖的影响。【方法】MTT法检测不同因子(NGF各浓度组、NGF+K252a各浓度组、bFGF组、bFGF+K252a组和正常培养液组)对正常和缺氧条件下培养的HRCEC的影响。【结果】随NGF浓度增加(20、50、100ng/ml),HRCEC细胞数目明显增多(正常氧浓度NGF组分别为0.254±0.033、0.696±0.029、1.136±0.051;缺氧条件相应各组为0.422±0.036、0.798±0.044、1.376±0.052,P均<0.05)。随K252a浓度增加(50、100、200nmol/L),NGF+K252a组正常氧浓度时分别为0.864±0.067、0.496±0.025、0.202±0.078,缺氧条件时相应为1.042±0.047、0.700±0.065、0.401±0.078,较同浓度NGF(100ng/ml)组HRCEC细胞数目减少(P<0.05),随K252a浓度增加HRCEC细胞数目减少趋势愈加显著(P均<0.05)。【结论】NGF可促进HRCEC的增殖,该作用可被特异性TrkA阻断剂K252a所阻断。

【Objective】 To observe NGF on cultured human retinal vascular endothelial cells （HRCEC） proliferation.【Methods】 The MTT assay was used to analyze the impact of culture HRCEC on different factors （NGF concentration groups,NGF ＋ K252a concentration groups,bFGF group,bFGF ＋ K252a groups,the normal culture medium groups） in normal and hypoxic condition.【Results】 With the increase of NGF concentration （20,50,100 ng / mL）,HRCEC significantly increased （normal condition： 0.254 ± 0.033,0.696 ± 0.029,1.136 ± 0.051;hypoxic condition： 0.422 ± 0.036,0.798 ± 0.044,1.376 ± 0.052,P 0.05）.Compared NGF ＋ K252a group with the same concentration of NGF （100 ng / ml） group,HRCEC reduced （P 0.05）,with increasing the concentration of K252a （50,100,200 nmol / L）,the trend of HRCEC decreasing is become more significant （normal condition：0.864 ± 0.067,0.496 ± 0.025,0.202 ± 0.078;hypoxic condition：K252a 1.042 ± 0.047,0.700 ± 0.065,0.401 ± 0.078,P 0.05）.【Conclusion】 NGF can promote the proliferation of HRCEC,the effect could be specifically blocked by TrkA inhibitor K252a.