大鼠Parv中间神经元形态学特征及其在纹状体分布规律的探察

Exploration on Morphology of Parv Interneurons and Their Distribution in Striatum in Rats

【目的】纹状体Parv中间神经元被认为是连接纹状体传入和传出神经元之间的枢纽。本实验旨在观察证实纹状体Parv神经元胞体、树突和轴突的形态学特征和在纹状体的分布及其与纹状体不同区域之间的关系。【方法】对成年雄性SD大鼠常规灌注固定和取脑;半导体冰冻切片之后进行免疫组化PAP方法单标记和双标记;光镜观察阳性神经元的形态并进行计数和计算其百分比;应用SPSS软件统计处理。【结果】Parv阳性神经元在纹状体呈现散在不均匀分布,在纹状体背外侧区的分布多于其它区域(P<0.001);Parv阳性神经元在Matrix间区分布的百分比显著高于Patch间区(82.0%比18.0%,P<0.001);纹状体Parv阳性细胞为典型的中等大小的中间神经元(平均直径11.68μm),胞体呈多边形或卵圆形,外侧区胞体直径大于内侧区(P<0.01);阳性神经元树突密集,表面光滑和未见树突棘;神经元轴突细长,在纹状体可见其分支。【结论】Parv阳性中间神经元在纹状体背外侧区的密集分布、高百分比聚集于Matrix间区和典型的中间神经元特征,提示其可能对纹状体不同区域的投射神经元具有选择性和特异性作用。

【Objective】 Intrastriatal Parv interneurons are considered to be the junction between striatal input and output neurons.This study purposes to confirm the morphology of Parv neurons and their distribution in striatum.【Method】 Adult male SD rats were perfused and the brains were removed.The sections were conducted with a semiconductor-frozen microtome,then single labeling and double labeling were immunocytochemically conducted with PAP technique.Positive neurons were observed,counted,and calculated,and analyzed with SPSS.【Results】 The distribution of Parv positive neurons in striatum was inhomogeneous,and was the most dense in the dorsal lateral area （P 0.001）;the distribution of Parv positive neurons in matrix compartment was apparently more than patch compartment （82.0% vs 18.0%,P 0.001）;Parv positive neurons in striatum were medium-sized cell bodies with polygonal or oval in shape （mean diameter is 11.68 μm）,and were larger in the lateral area than that in the medial area （P 0.01）;the positive dendrites were dense and smooth without dendrite spines;the positive axons 1 were slender and their collaterals extended in striatum.【Conclusion】 The characteristic of Parv positive neurons collecting mostly in lateral striatum and matrix compartment,as well as their traits of interneurons,indicated that they would affect the striatal projection neuronal function.