哮喘患儿急性发作期CD4^+CD25^+调节性T细胞水平和体质量指数的关系

Changes of CD4^+CD25^+ Regulatory T Cells in Peripheral Blood and Their Relation with Their Body Mass Index of Children with Acute Attack Asthma

【目的】探讨急性发作期哮喘患儿外周血CD4+CD25+调节性T细胞(Tr)的变化及其与体质量指数(BMI)的关系。【方法】外周血来自70例哮喘发作组患儿、30例缓解组和50例正常对照组小儿。将哮喘发作组再分为体质量正常组(40例)和超重组(30例)。应用流式细胞术检测患儿外周血的Tr细胞数变化,并计算患儿的BMI。【结果】急性发作期组患儿外周血Tr水平[(6.17±1.72)%]明显低于缓解期组患儿[(7.56±1.48)%]和对照组儿童[(7.13±1.48)%](P<0.05),而缓解期患儿外周血Tr水平与对照组儿童无明显差异(P>0.05)。正常体质量哮喘患儿CD4+CD25+Tr水平[(6.34±1.71)%]明显高于超重患儿[(4.74±1.20)%](P<0.05)。哮喘组患儿外周血CD4+CD25+Tr水平[(6.17±1.72)%]与其BMI水平(16.00±2.14)呈显著的负相关(rp=-0.814,P<0.05)。【结论】外周血Tr水平在哮喘患儿发作期显著降低,而在超重的患儿更低,且其水平与患儿的BMI呈显著的负相关。

【Objective】 To investigate the changes of CD4＋ CD25＋ regulatory T cells （Tr） in peripheral blood and their relation with their body mass index （BMI） of children with acute attack asthma.【Methods】 Peripheral blood was obtained from 70 children with acute attack asthma,30 remission children,and 50 normal control children.Then 70 children with acute attack asthma,were divided by normal weight group （40 cases） and overweight group （30 cases）.The levels of CD4＋ CD25＋ Tr of the patients were tested by flow cytometry （FCM）,and their BMI were calculated.【Results】 The levels of CD4＋ CD25＋ Tr [（6.17 ± 1.72）%] in acute attack group were lower than that in remission group [（7.56 ± 1.48）%] or that in the control group [（7.13 ± 1.48）%] （P 0.05）,but no difference between that in the remission and that in the control （P 0.05）.The CD4＋ CD25＋ Tr of asthmatic children with normal weight [（6.34 ± 1.71）%] was higher than that of asthmatic children with overweight [（4.74 ± 1.20）%] （P 0.05）.There was a remarkably negative correlation between the level of CD4＋ CD25＋ Tr of asthmatic children [（6.17 ± 1.72）% ] and the BMI （16.00 ± 2.14） （rp = -0.814,P 0.05）.【Conclusion】 The levels of CD4 ＋ CD25 ＋ Tr are remarkable decrease in attack asthmatic children,and more decrease in overweight patients.There is remarkably negative correlation between the levels of CD4＋CD25＋ Tr in peripheral blood of attack asthmatic children and their BMI.