双氢青蒿素在单壁碳纳米管修饰电极的伏安行为与测定

Determination and voltammetric behaviours of dihydroartemisinin on single-wall carbon nanotube-modified glassy carbon electrode

目的:研究双氢青蒿素在单壁碳纳米管修饰电极上的电化学行为及测定。方法:制备单壁碳纳米管(SWCNT)修饰玻碳电极,并用扫描电子显微(SEM)法、伏安法、电化学交流阻抗法对所制备的修饰电极进行表征。以此为工作电极,用线性扫描伏安法测定双氢青蒿素。结果:在pH=7.0,0.1mol·L-1的磷酸盐缓冲溶液(PBS)中,5.0×10-4mol·L-1双氢青蒿素在单壁碳纳米管修饰玻碳电极上还原峰电位为-0.85V,比同浓度双氢青蒿素在裸玻碳电极上的峰电位正移了270mV,峰电流显著增大。在优化实验条件下,还原峰电流与双氢青蒿素浓度在5.0×10-7～5.0×10-4mol·L-1的范围内呈线性关系,检出限为3.0×10-7mol·L-1。对5.0×10-4mol·L-1的双氢青蒿素平行测定10次的RSD为1.7%。结论:该方法简单、快速、灵敏,可用于含双氢青蒿素样品的含量测定。

Objective：To fabricate single-wall carbon nanotube（SWCNT）modified glassy carbon electrode and study the electrochemical behavior of dihydroartemisinin（DHA）and its determination at the SWCNT modified glassy carbon electrode.Methods：Cyclic voltammetry（CV）,scanning electron micro-scopy（SEM）,and linear sweep voltammetry（LSV）.Results：The reduction peak current of dihydroartemisinin is enhanced significantly at the SWCNT modified glassy carbon electrode（GCE）,in contrast to that at the bare GCE.Under the optimized experimental conditions,the reduction peak current of DHA on the SWCNT modified electrode was enhanced greatly.The good current-concentration relation was found over rang 5.0×10-7-5.0×10-4 mol·L-1with a limit detection of 3.0×10-7 mol·L-1 at optional conditions.Conclusion：The modified electrode was found to be sensitive and fast for the determination of dihydroartemisinin.