c-myc反义RNA对人肝癌细胞系HepG2的体外抑制作用

Inhibitory effect of expression of c-myc antisense RNA on human hepatocarcinoma cell line HepG2 in vitro

目的:探讨c-myc反义RNA对人肝癌细胞系HepG2的抑制作用。方法:用四元复合体基因转移系统将重组c-myc反义RNA表达载体pcDNA3.1-as-c-myc体外瞬时转染HepG2受体细胞,3d后流式细胞仪检测肝癌细胞内靶基因表达、细胞凋亡率并分析细胞周期。c-myc反义RNA转染人肝癌细胞HepG2,筛选稳定表达转染质粒的细胞(HepG2/as-c-myc)接种96孔板,绘制生长曲线。结果:c-myc反义RNA可显著降低细胞c-myc蛋白的表达水平,使细胞生长停滞于G0/G1期。稳定表达反义RNA的细胞生长速度与亲本细胞相比明显减慢,有显著性差异。结论:c-myc反义RNA可有效降低人肝癌细胞系HepG2的c-myc蛋白的表达,出现G0/G1期停滞,抑制肝癌细胞的生长增殖。

Objective：To study the inhibitory effects of c-myc antisense RNA on human hepatocarcinoma cells HepG2 in vitro.Methods：Three days after the four element complex with c-myc antisense RNA for transient transfection,the expression of c-myc protein,cell cycle and cell apoptosis were assayed by flow cytometry.Established hepato carcinoma cell line which could express c-myc antisense RNA stably.By growth curve, to study the biological effects on HepG2/as-c-myc cells which could express c-myc antisense RNA stably.Results：c-myc antisense RNA caused the expression of c-myc protein of HepG2 cells diminish;induced cell apoptosis and effected cell cycle pause at G0/G1 phase.Study of HepG2/as-c-myc cells,which could express c-myc antisense RNA stably,revealed that c-myc antisene RNA could inhibit cell growth.Conclusion：c-myc antisene RNA could effectively reduce the expression of c-myc protein on human hepatocarcinoma cells HepG2,induce cell cycle pause at G0/G1 phase and inhibit the growth of hepatocarcinoma cells.