赤芍药材辐照前后芍药苷含量变化研究

Research on the paeoniflorin content change in medicine of Paeonia lactiflora Pall.before and after radiation of Co-60 rays

目的:研究赤芍药材经钴-60射线辐照前后有效成分的含量变化情况。方法:采用HPLC方法,色谱柱为瑞士SPHERI-GEL ODS C18(150mm×4.6mm,5μm)柱,流动相为甲醇-水,梯度洗脱(0～10min,15%→20%甲醇;10～30min,20%→30%甲醇),流速1.0mL·min-1,检测波长为260.5nm,柱温25℃,分析时间为30min。结果:赤芍药材经钴-60射线辐照后主要有效成分降低近19%。结论:经过系统的方法学考察,该方法具有简便、稳定、可重复性强等特点。可作为赤芍药材辐照前后芍药苷含量变化的评价依据,同时在处理含赤芍药材的中药制剂时应考虑到辐照对主成分的影响。

Objective：To study the chang of the effective contents in Paeonia lactiflora Pall.befor and after the radiation of Co-60 rays.Methods：The HPLC was adopted.The separation was performed on a Switzerland SPHERIGEL ODS C18（150 mm×4.6mm,5 μm）column with methanol-water as mobile phase in gradient elution（0-10min,15%→20% methanol;10-30 min,20%→30% methanol）.The flow rate was 1.0 mL·min-1,the detection wavelength was set at 260.5nm,and the column temperature was maintaned at 25 ℃.Analysis time was 30 minutes..Results：The effective contents in Paeonia lactiflora had been reduced nearly 20% after the radiation of Co-60 rays.Conclusion：By investigating with systematic methodology,the techcique is convenient,stable and repeatable.It can be regarded as a standard in evaluating the content of paeoniflorin in Paeonia lactiflora before and after the irradiation of Co-60 rays.It also is a reference to the experiments of irradiating Chinese medical herbs.