摘要
目的:建立含大黄、黄芩、黄连和黄柏药材的中成药微生物限度检查方法。方法:通过对大黄、黄芩、黄连和黄柏4种药材的抗菌谱、最小抑菌浓度、敏感菌株等的归纳,总结出表征成药总抑菌活力的公式。结果:根据抑菌药材在处方中的含量可以快速计算成药的总抑菌活力,进而快速选择适宜的微生物限度检查法进行实验验证。结论:该方法可以大大简化实验操作,缩短工作时间,为简化2010年版中国药典的修订与验证提供了新的思路。
Objective：To establish a method of microbial limit test of Chinese medicines which include Rhubarb,baical skullcap root,Golden Thread and Amur Cork-tree.Method：To gain a dynamic formula for the characterization of bacteriostasis through the summary of antibacterial spectrum,the minimum inhibitory concentration and the sensitive strains of Rhubarb,baical skullcap root,Golden Thread and Amur Cork-tree.Result：In accordance with the amount of antibacterial medicines in the prescription of medicines,we can quickly calculate the total antibacterial activity,then choose suitable microbial limit Test for experimental verification as soon as possible.Conclusion： The method can greatly simplify the experiment and shorten working hours,which provide a new way of thinking to simplify the amendment and the verification of Chinese Pharmacopoeia of 2010 version.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第3期558-562,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
微生物限度检查法
中成药
大黄
黄芩
黄连
黄柏
抑菌活力
microbial limit test
Chinese medicines
Rhubarb
baical skullcap root
Golden Thread
Amur Cork-tree
antibacterial activity
