5-脱氧杂氮胞苷对胰腺癌细胞Runx3表达的影响

Effects of 5-Aza-2deoxycytidine on the expression of Runx3 in pancreatic cancer cell line

目的:研究5-脱氧杂氮胞苷对人胰腺癌细胞Panc-1中Runx3基因表达的影响并探讨其机理。方法:培养胰腺癌细胞株Panc-1,用5-脱氧杂氮胞苷处理,处理前后采用流式细胞仪、RT-PCR方法检测Runx3基因的表达情况。建立裸鼠人胰腺癌细胞Panc-1皮下移植瘤模型,观察裸鼠致瘤性变化。结果:经5-脱氧杂氮胞苷处理后的胰腺癌细胞株Panc-1中Runx3蛋白表达增强,裸鼠致瘤性降低。结论:Runx3蛋白的表达增强可能与DNA甲基转移酶抑制剂5-脱氧杂氮胞苷抑制Runx3启动子区域的高甲基化有关,并抑制胰腺癌细胞一Panc-1在体内、体外的生长,这可能成为一条胰腺癌治疗的新思路。

Objective：To study the effects of expression of 5-Aza-2deoxycytidine on pancreatic cancer cell line Panc-1 and to explore its elements.Methods：The cultured pancreatic cancer cell line Panc-1 was treated with 5-Aza-2deoxycytidine.The expression of Runx3 gene was detected by flow cytometry,RT-PCR before and after treatment.The models of human pancreatic cancer line Panc-1 transplanted subcutaneously were established in nude mice and to observe the changes in tumori-genicity in nude mice.Results：The expression of Runx3 protein in pancreatic cancer cell line Panc-1 of 5-Aza-2deoxycytidinetreatment increased.The tumorigenicity in nude mice decreased. Conclusion：These but also in inhibit the gro results suggested that 5-Aza-2deoxycytidine may not only induce the increasing expression of Runx3 protein by reactive Runx3 gene silenced by promoter hypermethylation,wth of pancreatic cancer cell line Panc-1 in vivo and in vitro,which can be a new approach for treatment of pancreatic cancer.