海洛因依赖对大鼠甲状腺降钙素阳性细胞及血清FT_3、FT_4、促甲状腺素的影响

Effects of heroin dependence on rat’s calcitonin positive cells in thyroid gland and serum FT_3,FT_4,TSH

目的探讨海洛因依赖对大鼠甲状腺C细胞表达降钙素(CT)及血清FT3、FT4、促甲状腺素(TSH)的影响。方法成年雄性SD大鼠55只,随机分为正常对照组、盐水对照组及海洛因依赖组。皮下注射海洛因建立海洛因依赖大鼠模型,分别于模型建立的第10、17、24、31、38天取甲状腺组织。采用免疫组织化学SABC法、细胞计数、图像分析方法及化学发光法技术进行研究。结果与正常对照组及生理盐水对照组比较,海洛因依赖组大鼠甲状腺内CT阳性细胞免疫染色强度减弱,细胞数量减少(P<0.05);平均灰度值显著升高(P<0.05),且呈逐渐增高趋势。海洛因依赖期间,血清TSH浓度与正常对照组比较,差异无统计学意义(P<0.05)。海洛因依赖10、17d,血清FT3浓度较正常对照组降低(P<0.05)。海洛因依赖期间,血清FT4浓度较正常对照组有所升高,在24d和38d增高明显(P<0.05)。结论海洛因依赖期间,大鼠甲状腺CT阳性细胞数量减少,分泌功能减退。血清FT3浓度有所降低,FT4浓度有所升高,TSH浓度无明显变化,提示海洛因依赖可能使甲状腺功能受到影响。

Objective To investigate ealeitonin（CT） and serum FT3, FT4, TSH the effect of the heroin addicts on rat＇s thyroid C cells, the secretion of Methods Adulthood 55 male SD rats were randomly divided into normal control group（NCG） , saline control group（SCG） and heroin dependence group（HDG）. Heroin-dependent rat models were set up by the way of subcutaneous injection of heroin and the thyroid glands were excised on the 10th, 17tb, 24th, 31st, and 38th days after models were set up. Tissues were assessed by immunohistochemical SABC method, cell counting, image analysis method and chemiluminescence techniques. Result Compared with the normal control group（NCG）and saliane control group （SCG） , the immune staining intensity was weakened; the number of CT positive cells was reduced （P 〈 0. 05 ） ; the average gray value was significantly higher（ P 〈 0. 05 ） and showed a gradual increase in the CT positive cells of heroin dependence group （ HGD）. The concentration of TSH in HDG was not statistically significant as compared with the NCG. The concentration of FT3 in HDG decreased gradually on the 10th day and the 17th day as compared with the NCG. （ P 〈 0.05）. The concentration of FT4 in HDG increased gradually on the 24th day and the 38th day as compared with the NCG. （P 〈 0. 05）. Conclusion During heroin dependence period, the number and functional of CT positive ceils has decreased. The changes of concentration of FT3 and FT4 suggest that the function of thyroid gland has changed.