摘要
目的用异硫氰荧光素(FITC)-右旋糖苷和四甲基若丹明(TMR)-右旋糖苷标记小鼠2-细胞胚胎的两个卵裂球,观察其发育,以探讨囊胚Em-Ab极性的形成。方法向受精卵注射FITC-右旋糖苷确定标记物对胚胎的伤害性,向2-细胞胚胎两个卵裂球分别注射FITC-右旋糖苷和TMR-右旋糖苷,将标记后的胚胎体外培养发育至囊胚,观测两个卵裂球后代在囊胚中的分布情况。结果2-细胞胚胎两个卵裂球的后代在囊胚中分布并无规律。结论2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分。
Objective Kunming strain（KM） mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine（TMR） and fluorecein isothiocyante （FITC）was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2- cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected. Conclusion The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第1期124-127,共4页
Acta Anatomica Sinica
基金
黑龙江省教育厅自然科学厅面上项目(11531028)
