摘要
用原位核酸末端杂交和免疫细胞化学技术,分别观察28例16～32周人胎肺组织不同发育阶段的增殖与凋亡的表达特征。实验结果表明:支气管粘膜上皮细胞在胎肺发育的早、中期(即假腺期和小管期),细胞的凋亡与增殖均明显较晚期旺盛,而且两者均于胎肺发育的小管期达到最高峰;但凋亡与增殖细胞在不同发育时期各有不同的定位,提示细胞的凋亡与增殖行为在胎肺管腔发生及分支延伸过程中有不同作用。
28 cases of human fetal lung tissues (from 16-32 weeks) were studied by use of in situ hybridization and immuocytochemistry technique to examine the expressive characteristics of cell apoptosis and proliferating cell nuclear antigen (PCNA) in bronchial epithelial cells during the developmental stages. The results demonstrated: In the early and middle stages of the fetal lung (the pseudoglandular stage and the canalicular stage), cell apoptosis and proliferation of the bronchial epithelial were obviously more active than that of in the late stage (the terminal sac stage), and both the expressive rate reached the peak at the canalicular stage; however, the positive reactions were respectively different at individual developmental stages and in different locations. The results suggested that the activities of cell apoptosis and cell proliferation played different roles in the developmental process of cavitation and arborization in human fetal lung.
基金
国家自然科学基金资助项目(批准号39770300)
关键词
胎肺
免疫细胞化学
细胞增殖
细胞凋亡
发育
Fetal lung
Proliferation
Apoptosis
In situ hybridization
Immunocyto- chemistry
