环氧化酶-2和S-100^+树突状细胞在喉癌中的表达及意义

Expression and significance of COX-2 and S-100 positive dendritic cell in laryngeal carcinoma

目的:探讨环氧化酶-2(COX-2)和S-100+树突状细胞在喉癌组织中的表达及临床意义。方法:采用免疫组织化学SP法检测65例喉癌组织及34例癌旁阴性喉黏膜组织COX-2和S-100+树突状细胞表达,并分析它们与临床病理参数及预后的关系。结果:COX-2在喉癌的表达率为63.08%(41/65),对照组为14.70%(5/34),两者比较差异有统计学意义(P<0.05)。COX-2表达与T分期、临床分期有关(均P<0.05)。S-100+树突状细胞在喉癌中表达率为61.54%(40/65),对照组为0,两者比较差异有统计学意义(P<0.05)。S-100+树突状细胞表达与喉癌临床分期、淋巴结转移比较差异均有统计学意义(均P<0.05)。Kaplan-Meier生存分析显示COX-2、S-100+树突状细胞表达与患者无瘤生存率、总生存率有密切关系(P<0.05)。Cox回归分析显示,S-100+树突状细胞是影响预后的独立危险因素(P<0.05)。结论:喉鳞状细胞癌组织中存在COX-2和S-100+树突状细胞高表达,两者与喉癌的发生发展有关,可作为判断喉癌预后的重要指标。

Objective：To explore the expression of cyclooxygenase-2（COX-2） and S-100 positive dendritic cell in laryngeal carcinoma tissue and their clinical significance.Method：Sixty-five samples of laryngeal carcinoma and thirty-four biopsies of adjacent noncancerous tissue were obtained. Immunohistochemical technique（SP method） was used to detect the expression of COX-2 and S-100 positive dendritic cell,and the relationship of their expression with clinical pathological parameters and prognosis was analyzed.Result：The rates of COX-2 expression were 63.08%（41/65）and 14.70%（5/34）in laryngeal carcinoma and control group,respectively. The difference was significant（P〈0.05）.The positive expression of COX-2 was correlated with T and clinical stage in laryngeal carcinoma（all P〈0.05）.The rates of S-100 positive dendritic cell expression were 61.54%（40/65）and 0 in laryngeal carcinoma and control group,respectively. The difference was significant（P〈0.05）.S-100 positive dendritic cells showed significant differences between early and late clinical stage and lymph node metastasis （all P〈0.05）.Kaplan-Meier survival analysis showed that patients with positive expression of COX-2 and S-100 positive dendritic cell had worse disease-free and overall survival（P〈0.05）.Cox regression analysis showed that S-100 positive dendritic cell was indicated as an independent prognostic factor for survival（P〈0.05）.Conclusion：COX-2 and S-100 positive dendritic cell are highly expressed in laryngeal carcinoma tissue. It suggests that the expression of COX-2 and S-100 positive dendritic cell is related to the process of carcinogenesis and may be the important indicators in laryngeal carcinoma for prognosis.