阻塞性睡眠呼吸暂停低通气综合征上气道开大肌凋亡现象研究

Research on apoptosis changes of tensor veli palatini in patients witho bstructive sleep apnea-hypopnea syndrome

目的:观察阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者腭帆张肌中Bax、Bcl-2的表达水平,探讨OSAHS患者上气道开大肌肌纤维是否存在凋亡现象。方法:选取30例OSAHS患者作为实验组,10例已排除OSAHS的慢性扁桃体炎患者作为对照组。采用免疫组织化学Elivison二步法和计算机图像分析系统检测两组腭帆张肌中Bax、Bcl-2的表达水平并进行对比分析。结果:①实验组Bax的表达水平和Bax/Bcl-2的比值明显高于对照组,差异有统计学意义(P<0.05);②Bax的表达水平与呼吸紊乱指数呈正相关(r=0.697,P<0.01)。结论:OSAHS患者上气道开大肌存在凋亡现象,且病情越重凋亡的程度也越严重。

Objective：To explore apoptosis changes of dilator muscles in the upper airway by detecting the expression of Bax,Bcl-2 in tensor veli palatini in patients with OSAHS.Method：The expression of Bax and Bcl-2 were detected in tensor veli palatini in 30 cases with OSAHS and 10 cases chronic tonsillitis without OSAHS by immunohistochemistry and image analytical system,and the results were analyzed.Result：①The expression levels of Bax in the OSAHS group increased significantly compared to control group（P〈0.05）,but there were no significant differences of Bcl-2 expression between two groups,the ratio of Bax/Bcl-2 increased significantly（P〈0.05）. ②There were positive correlations between AHI and the expression levels of Bax（r=0.697,P〈0.01） respectively in the test group.Conclusion：The results indicate that apoptosis occurred in tensor veli palatini in patients with OSAHS,and the more severity of OSAHS ,the more apoptosis.