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Stat3反义核苷酸通过调节凋亡相关因子诱导喉癌细胞凋亡 被引量：3

Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors
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摘要 目的:探讨反义Stat3基因诱导喉癌细胞凋亡的机制。方法:将设计好的已证实能诱导人喉癌细胞凋亡的Stat3反义寡核苷酸序列,应用脂质体瞬时转染法转染人喉癌Hep-2细胞,应用Western blot、PCR检测Hep-2细胞中Bcl-2,Bax及C-Myc基因及蛋白的表达情况。结果:Western blot和RT-PCR结果显示转染Stat3反义寡核苷酸细胞组,Bax的表达随反义寡核苷酸浓度增加,表达增强,而Bcl-2及C-Myc的表达则减弱。结论:反义Stat3寡核苷酸通过上调Bax,下调Bcl-2及C-Myc基因表达来参与其诱导人喉癌Hep-2细胞凋亡的过程。 Objective：To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide （ASODN）.Method：The designed Stat3 ASODN was transferred into laryngeal cacinoma Hep-2 cell by lipofection. Expression of Bcl-2,Bax and C-Myc were detected by Western blot and PCR.Result：Western blot and PCR results demonstrated that Stat3 ASODN could significantly increased the expression of Bax and decreased the expression of Bcl-2 and C-Myc when the concentration of antisense oligodeoxynucleotide were heightened.Conclusion：Stat3 ASODN participate in apoptosis by enhancing the expression of Bax and reducing the expression of Bcl-2 and C-Myc.
作者 吕海丽 张秋航 严波
机构地区 首都医科大学宣武医院耳鼻咽喉-头颈外科
出处 《临床耳鼻咽喉头颈外科杂志》 CAS CSCD 北大核心 2010年第4期155-157,共3页 Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词 喉肿瘤 反义寡脱氧核苷酸 STAT3 凋亡机制 laryngeal neoplasms antisense oligodeoxynucleotide Stat3 apoptosis mechanism
分类号 R739.65 [医药卫生—肿瘤]
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参考文献8

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二级参考文献1

共引文献5

同被引文献22

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临床耳鼻咽喉头颈外科杂志
2010年 第4期

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