SKP2及MRP-1/CD9在声门癌及癌旁组织中的表达及其临床意义

Clinical significance of SKP2 and MRP-1/CD9 expression in glottic carcinoma and adjacent tissues

目的:观察声门癌及癌旁组织中SKP2及MRP-1/CD9蛋白的变化意义及其对声门癌手术安全切除范围的指导意义。方法:用免疫组织化学SP法检测38例声门鳞状细胞癌组织及距癌组织边缘2、5、10mm的组织中SKP2及MRP-1/CD9蛋白的表达,并取10例声带息肉组织作对照。结果:SKP2蛋白产物异常表达按息肉黏膜组织、癌旁组织(2、5、10mm)及癌组织的顺序依次递减,差异有统计学意义(均P<0.05);MRP-1/CD9蛋白产物异常表达按息肉黏膜组织、癌旁组织(2、5、10mm)及癌组织的顺序依次递增,差异有统计学意义(均P<0.05)。结论:SKP2及MRP-1/CD9均可作为判断喉癌生物学特性的参考指标,声门癌手术安全切缘以距肿瘤≥5mm的标准较为适宜。

Objective：To study the expression of SKP2 and MRP-1/CD9 protein in glottic cancer and adjacent tissues,and to analyze its significance for a safe surgical margin.Method：Thirty-eight cases of glottic squamous cell carcinoma were studied for its cancer tissue,tissue 2 mm,5 mm ,and 10 mm away from cancer ,and 10 cases of vocal cord polyp were served as control. SKP2 and MRP-1/CD9 protein were examined by immunoh istochemical method.Result：The positive expression of SKP2 proteins decreased in sequence of polyp mucosa,those adjacent to carcinoma （10 mm,5 mm,2 mm ） and cancer tissue,and there was significant difference between them（P〈0.05 ）;On the contrary,the positive expression of the MRP-1/CD9 proteins increased in sequence of polypusmucosa,those adjacent to carcinoma （10 mm,5 mm,2 mm） and cancer tissue,and there was significant difference between them （P〈0.05）.Conclusion：SKP2 and MRP-1/CD may act as the reference index for judging the biological speciality of LSCC. It is appropriate to regard 5 mm or above 5 mm away from tumors as a safe margin for surgical treatment of glottic carcinoma.