苦参碱诱导人肺鳞癌SK-MES-1细胞凋亡作用及其可能机制

Matrine-induced apoptosis in human lung squamous cell carcinoma SK-MES-1 cells and the possible mechanism

目的:探讨苦参碱(MT)诱导人肺鳞癌细胞株SK-MES-1凋亡作用及其抗肿瘤机制。方法:培养人肺鳞癌细胞株SK-MES-1,用不同浓度的MT处理SK-MES-1细胞,用MTT法检测MT对SK-MES-1细胞生长增殖的抑制作用;流式细胞术检测细胞凋亡率和细胞周期;流式细胞术检测细胞相关凋亡蛋白Bcl-2、bax、caspase-3表达。结果:MT抑制SK-MES-1细胞增殖和诱导SK-MES-1细胞的凋亡作用均呈剂量和时间依赖性。与对照组相比,苦参碱处理后的SK-MES-1细胞G0/G1期百分比明显增高,S期细胞比例下降。Bax和capase-3表达增强,Bcl-2表达下调。结论:MT在体外能抑制人肺鳞癌细胞株SK-MES-1增殖,诱导细胞凋亡,其诱导作用具有明显的量效和时效关系,可能与抑制Bcl-2活性、激活bax和capase-3有关。

Objective：To explore the effects of matrine on the cell cycle and apoptosis in human lung squamous cell carcinoma SK-MES-1 cells and explore the possible mechanisms.Methods：The effect of matrine on cell proliferation was assessed using MTT assay.The cell cycle arrest and the apoptosis rate induced by matrine were determined with flow cytometry.The expression of caspase-3,Bcl-2 and Bax proteins were assayed by flow cytometry.Results：Matrine inhibited the proliferation of SK-MES-1 cells in a dose-and time-dependent manner.Compared with the control group,the matrine-treated cells showed increased cell percentage arrested in G0/G1 phase with decreased S-phase cells.After treatment of 48 hours,the apoptosis rate was increased.The expression of caspase-3 and Bax increased.The expression of Bcl-2 decreased.Conclusion：Matrine can inhibit SK-MES-1 cell proliferation,cause cell cycle arrest at G0/G1 phase,and induce apoptosis in a dose-and time-dependent manner,which may be related to activation of caspase-3 and Bax and suppression of Bcl-2.