摘要
目的建立祖师麻注射液中紫丁香苷、祖师麻甲素的含量测定方法。方法使用Agilent ZORBAXSB-C18色谱柱,流动相:乙腈-0.05%磷酸溶液进行梯度洗脱,变化检测波长223、327nm,流速为1.00mL.min-1,柱温为30℃。结果利用2004年A版《中药色谱指纹图谱相似度评价系统》计算软件生成祖师麻注射液指纹图谱,共有17个特征峰,各峰分离度良好,符合指纹图谱检测要求。结论采用HPLC指纹图谱技术可以有效地控制祖师麻注射液的质量。
Objective To study and establish the fingerprint chromatogram of Zushima Injecton.Methods Agilent ZORBAX SB-C18 at 30℃.The mobile phase was consisted of acetonitrile-0.05% phosphoric acid with a gradient of the solvent minxtures,and with change of detection wavelength at 223nm and 327nm.The flow rate was 1.00mL·min-1.Results The standard fingerprints of Zushima Injection was originated from ＂The Similarity Evaluation for Chromatographic Fingerprint of TCM＂（Version 2004 A）,and 17 common peaks existed in the fingerprint.Each was accuracy,stability and repeatability,and satisfied to the requirements of fingerprint.Conclusion The HPLC fingerprint method can be used in the quality control of Zushima Injection.
出处
《中国药事》
CAS
2010年第3期289-292,共4页
Chinese Pharmaceutical Affairs
