芪薏益肝煎对小鼠H22移植瘤PTEN表达的影响

Effect of Qiyi Yiganjian on expression of PTEN gene of H22 carcinoma grafted in mice

目的:探讨芪薏益肝煎对小鼠H22移植瘤的抑瘤作用及作用机制。方法:小鼠右腋下皮下种瘤造成实体瘤模型,将30只小鼠分为三组,即对照组、芪薏益肝煎组和5-Fu组,每组各10只,连续用药15 d。第16天处死小鼠,计算胸腺指数、脾指数;剥离瘤块秤重并计算抑瘤率;采用免疫组化技术检测抑癌基因(PTEN)的表达。结果:芪薏益肝煎组小鼠皮下肿瘤重量低于生理盐水组,PTEN表达高于生理盐水组,有非常显著性差异(P<0.01),并且其抑瘤率为37.00%。结论:芪薏益肝煎对小鼠肝癌细胞具有明显抑制作用,其作用机制与PTEN表达有关。

Objective：To discuss the anti-tumor effect and mechanism of Qiyi Yiganjian on H22 carcinoma grafted in mice.Methods：After injecting ascites hepatoma cell line H22 ceils into their right axial,30 mice were divided into three groups：control group,Qiyi Yiganjian group and 5-Fu group.The tumor-bearing mice received corresponding treatments for 15 consecutive days,after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices.The tumor mass was also weighed and the tumor inhibition rate calculated.PTEN was examined by using immunohistochemistry straining method in the tumor tissue.Results：The subcutaneous tumor weight in Qiyi Yiganjian group lower than those in normal saline group.The PTEN expression in Qiyi Yiganjian group was significantly higher than that in normal saline group which inhibited tumor growth by 37.00%（P 〈0.01）.Conclusion：The mechanism of Qiyi Yiganjian significantly inhibiting effect on ascites hepatoma cell line H22 ceils is relative to expressions of PTEN.