摘要
提出了石墨炉原子吸收光谱法测定顺铂人血浆中铂浓度。采集人静脉血作样品,用浓硝酸及过氧化氢消解。冷却至4℃,使脂肪固化并过滤除去。收集滤液,蒸干,用200μL盐酸(1+99)溶液浸取残渣,所得溶液供石墨炉原子吸收光谱分析。方法的线性范围为0.02～1.00mg.L-1,检出限(3σ)为0.016mg·mL-1,回收率在94.6%～120.0%之间,相对标准偏差(n=5)小于7%。
A method for determination of platinum in human plasma released from the sustained-release implant of cis-platinum by GF-AAS was proposed. Veinous blood was taken as sample and digested with conc. HNO3 and H2O2. The clear digested solution was cooled to 4 ℃to solidify fat which was then separated by filtration. The filtrate was collected and evaporated to dryness, the residue was taken up with 200 μL of HCl（1＋99） and used for GF-AAS analysis under the optimized conditions. Linear relationship was obtained between values of absorbance and mass concentration of Pt（Ⅳ） in the range from 0.02-1.00 mg·L-1 Detection limit （3a） of the method was found to be 0. 016mg·L-1 Recovery and precision were tested on the base of simulated samples by standard addition method, giving values of recovery in the range from 94. 6% to 120. 0%, and values of RSD＇s （n= 5） less than 7%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第3期257-259,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
安徽省十一五科技攻关重点项目(06013062A)
