分光光度法测定当归补血汤及其组分单味药提取液的抗氧化能力

Spectrophotometric Determination of Anti-oxidation Capacity of Extract of Dangguibuxuetang Decoction and the 2 Componental Single Medicinal Herbs

对复方当归补血汤及其组成单味药即当归和黄芪的水提取液,以及两者的单味药的水提取液及乙醇提取液的抗氧化能力作了比较研究。将由Fenton反应产生的羟自由基在pH4.6乙酸盐缓冲溶液与水杨酸反应,生成紫红色的2,3-二羟基苯甲酸,其吸收峰在510nm波长处。当有任一抗氧化组分存在于此反应体系时,由于其与反应体系中的羟自由基的优先反应,使此体系在510nm处的吸光度下降。据此,对上述药物提取液的抗氧化能力进行了试验。所得结果表明:上述药物的提取液均呈良好的抗氧化能力(即清除羟自由基的能力),而复方当归补血汤中主成分药物的提取液(无论是水或乙醇的提取液)的抗氧化能力均高于单味药物的提取液。比较水提取液与乙醇提取液的抗氧化能力的结果表明,前者的效果更好。

A comparative study on the anti-oxidation capacity of aqueous and ethanol extracts containing angelica and astragalus membranaceus of the Dangguibuxuetang decoction and the 2 componental single herbs was made. Hydroxyl free radical produced by the Fenton reaction was reacted with salicylic acid in an acetate buffer medium of pH 4. 6, to form a reddish violet product, 2, 3-dihydroxybenzoic acid with its absorption maximum at 510 nm, where the absorbance was measured. When any of the anti-oxidative ingredient in the medicines was added to the above mentioned reaction system, decrease of absorbance of the color reaction was observed due to the elimination of·OH group by the anti-oxidative ingredient. Based on these facts, the anti-oxidation capacity of the medicines was studied. As shown by the tested results, both the extracts of the compound haematic decoction and the single medicinal herbs showed good anti-oxidation capacity against the · OH free radical, however, higher antioxidation capacity was obtained with the extracts （either aqueous or ethanol） of the decoction. But as comparing the anti-oxidation capacity between the aqueous extract and ethanol extract, better results were found with the former.