哈萨克族居民高血压与ACE和AT_1R基因多态性

Relationship between angiotension converting enzyme gene and angiotension Ⅱ type 1 receptor gene polymorphism and essential hypertension in Kazakh of Xinjiang

目的探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性、血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与新疆哈萨克族居民原发性高血压关系。方法采用病例对照研究设计,以220例高血压患者为高血压组,220名血压正常者为对照组。应用聚合酶链反应和限制性酶切的方法检测ACE基因I/D与AT1R基因A1166C多态性。结果高血压组中,ACE I/D DD、ID、II 3种基因型的频率分别为43.64%,34.54%,21.82%,对照组中分别为27.72%,48.64%,23.64%,差异有统计学意义(χ2=19.89,P<0.01);高血压组中D和I 2种等位基因频率分别为60.91%,39.09%;对照组中分别为47.95%,52.05%,差异有统计学意义(χ2=14.89,P<0.01);高血压组中,AT1RA1166C AA和AC2种基因型的频率分别为78.6%,21.4%,对照组中分别为81.4%,18.6%,差异无统计学意义(χ2=0.51,P>0.05);高血压组中A和C2种等位基因频率分别为89.3%,10.7%,对照组中分别为90.7%,9.3%,差异无统计学意义(χ2=0.45,P>0.05)。结论当个体携带ACE基因DD基因型时,增加患高血压的危险性;AT1R基因A1166C多态性与哈萨克族居民高血压发生无关;ACE基因ATR基因之间可能有交互作用。

Objective To investigate the relationship between angiotension converting enzyme（ACE）gene insertion/deletion（I/D） polymorphism,angiotension Ⅱ type 1 receptor（AT1R）gene A1166C polymorphism and essential hypertension in Kazakh.Methods A case-control study was conducted in 220 cases and 220 controls.Polymerase chain reaction and restrict fragment length polymorphism（PCR-PELP） technique were used to detect the genotype polymorphism.Results Among the cases with hypertension,the I/D frenquencies of DD,ID,II were 43.64%,34.54%,21.82%,and that of the controls were 27.72%,48.64%,23.64%,respectively.There was a significant difference between the two groups（χ2=19.89,P0.01）.60.91% of D and 39.09% of I allele were distributed in hypertention cases,while 47.95% of D and 52.05% of I in controls with a significant difference between the two groups（χ2=14.89,P0.01）.Among the cases with hypertension,the A1166C frenquencies of AA and AC were 78.6%,21.4%,and that of the controls were 81.6%,18.4%,respectively,with no significant difference between the two groups（χ2=0.51,P0.05）.89.3% of A and 10.7% of C allele were distributed in hypertention cases,while 90.7% of A and 9.3% of C in controls and there was no significant difference between the two groups（χ2=0.45,P0.05）.Conclusion The DD and AA,AD and AC genotype of the gene might increase the risk of hypertension.There is no relationship between A1166C gene polymorphism of AT1R gene and hypertension in Kazakh people.There is an interaction between ACE gene and AT1R gene.