缺血性脑血管病患者载脂蛋白E基因多态性频率分布

Prevalence of apolipoprotein E gene polymorphism in ischemic cerebrovascular disease

为研究中国汉族缺血性脑血管病(ICVD)患者 ApoE 基因型及等位基因频率分布状况,随机选择 ICVD 患者93例,平均年龄63.9岁;并选择同龄非 ICVD 受检者100例作为对照。采用单链构象多态性(SSCP)技术检测 ApoE 基因型。主要结果如下:(1)ICVD 患者组 ApoEε_(3/3)频率明显低于同龄对照组(P<0.05),(2)中年组 ICVD 患者中ε_(3/3)为56.78%,明显低于同龄对照组之73.07%(P<0.05),ε_(4/3)频率(31.37%)较同龄对照组(11.54%)增高(P<0.05).ε_4等位基因频率(19.61%)显著高于同龄对照组(9.62%)(P<0.05)。本实验结果提示:ApoEε_4基因频率增高与ICVD 发病有关,似可作为 ICVD 的危险标志,但其与老年患者关系不明显。

In order to study the distribution of APO E gene polymorphism in the Han's pa- tients with ICVD,93 patients(mean age 63.9 years)were selected randomly and 100 age-matched cases without ICVD were selected as control group,Single-strand conformational polymorphism (SSCP)was used to determine the APO E genotype. The main results were as follows:(1)The frequency of ApoE ε_(3/3)genotype in ICVD group was lower than that in age-matched control significantly(P<0.05).(2)The freqnency of ε_(3/3)in middle age patient group was 56.87%,which was significantly lower than 73.07% of age-matched group,and the frequency of ε_(4/3)genotype(31.37%)and ε_4 allele(19.61%)were significantly high- er than that of age-matched controls(11.54% and 9.62%)respectively(P<0.05). The results indicated that high Aopε_4 allele frequency was associated with ICVD,which could be a genetic marker for ICVD.However.the relation was not significant in elderly patient.