不同训练方式对大鼠腓肠肌p53和IL-6的影响

Effects of Different Exercise Modes on P53 and IL-6 Expression of Gastrocnemius Muscle in Rats

目的：探讨不同训练方式对大鼠腓肠肌p53和IL-6的影响。方法：21只大鼠随机分为3组,即安静组（C,n=7）,耐力训练组（E,n=7）,间歇性冲刺训练组（S,n=7）。耐力训练组和间歇性冲刺训练组进行跑台训练8周;末次训练后24~48 h内,各组大鼠安静状态断颈处死,取腓肠肌。采用Elisa法分别测定腓肠肌中p53和IL-6水平。结果：1）E组（P〈0.05）和S组（P〈0.01）腓肠肌p53蛋白表达显著低于C组。2）E,S组腓肠肌IL-6表达与C组相比无显著性差异。结论：冲刺训练和耐力训练对P53的表达产生下调作用,在一定程度上可延缓骨骼肌衰老,同时间歇性运动效果更明显;而不同训练方式对IL-6的表达并未见有显著影响。

Objective： To investigate the effects of different exercise modes on p53 and IL-6 in rats＇gastrocnemius muscle.Methods：Twenty-one male SD rats arerandomly and equally divided into three groups： control group（C,n=7）,endurance exercise group（E,n=7）,and intermittent strenuous exercise group（S,n=7）.The rats are sampled after 8-week exercise training.Between 24h and 48h after the last training,the rats are killed,gastrocnemius muscle are excised quickly for ELISA.Results： 1） The P53 expression of gastrocnemius muscle is downregulated significantly in E（p0.05） and S（p0.01） group;2） The IL-6 expression of gastrocnemius muscle has no significant change in E and S group.Conclusion： The effects of endurance mode and intermittent strenuous mode exercise on p53 can delay aging of muscle;But the connection between the expression of IL-6 and different exercise modes is not clear yet.