猫杯状病毒全基因组的克隆及序列分析

Cloning and Sequence Analysis of Feline Calicivirus Complete Genome

采用RT-PCR和重组PCR扩增了猫杯状病毒(feline calicivirus,FCV)CH-GD株的全基因,并进行了序列测定,用DNAStar软件对其核苷酸序列和氨基酸序列进行比较分析。结果表明,首次分离于中国广东的CH-GD株与各参考毒株有较大的差异。CH-GD株与各参考毒株之间的同源性仅为75.4%~77.1%,明显低于各参考毒株之间的同源性(78.9%~99.9%)。同时遗传进化树显示,14个分离株形成两大分支,CH-GD株独自在一分支,各分离株无明显的地域性差异。对FCV衣壳蛋白6个区(A^F)的分析结果发现,CH-GD株中A^F区的特点与报道的相符。此外还发现,CH-GD株有3个区域的3个连续的氨基酸发生了变异,与参考毒株相比,CH-GD株在这3个区域的抗原性和亲水性也都发生了相应的变化。

Complete sequence of feline calicivirus（FCV） CH-GD strain was amplified by RT-PCR and recombinant PCR.DNAStar software was used to analyse nucleotide sequences and amino acid sequences.The homology of CH-GD strain which was isolated first in Guangdong province of China with reference strains was between 75.4% and 77.1%.It was lower than that was between reference strains（78.9% and 99.9%）.There were two branches between 14 strains.CH-GD strain was in one branch and there was no evident difference in district.Analysing six regions of FCV capsid protein,the characteristic of A-F region was equal to that had been reported.Antigenicity and hydrophilicity were different from reference strains in three regions where three consecutive amino acids were variation.