多浪羊高繁殖力候选基因FecX^G位点SSCP分析

Analysis of FecX^G as the Candidate Gene for Prolificacy in Duolang Sheep by SSCP

采用聚合酶链式反应—单链构象多态性分析技术(polymerase chain reaction-single strand conformation polymorphism,PCR-SSCP)技术研究绵羊高繁殖力候选基因FecXG位点在多浪羊中的单核苷酸多态性。试验结果表明:多浪羊中BMP15基因的FecXG位点并不存在多态性,推断FecXG位点与多浪羊多胎性状无关。

The single nucleotide polymorphism of FecXG in Duolang sheep was studied by polymerase chain reaction-single strand conformation polymorphism（PCR-SSCP）.The results showed that FecXG gene was not detected in Duolang sheep,so we infered that FecXG was not one of the major gene that affected the prolificacy in Duolang sheep.