延边黄牛PRKAG3基因第10外显子单核苷酸多态性及其与肉质性状的相关分析

Single Nucleotide Polymorphism in Yanbian Yellow Cattle Exon 10 of PRKAG3 Gene and its Association with Meat Quality Traits

采用PCR-SSCP方法对120头延边黄牛PRKAG3基因的多态性进行检测,结果发现第10外显子存在C4738T突变,产生3种基因型。不同基因型与肉质性状均值差异显著性检验结果发现,基因型CT与a2*4、b2*4、油酸存在一定的负相关(P<0.05);与嫩度、谷氨酸、精氨酸、丙氨酸、缬氨酸和脯氨酸有一定的正相关(P<0.05);基因型CC与天冬氨酸存在一定的正相关(P<0.05)。

The polymorphisms of PRKAG3 gene were detected by PCR-SSCP method in 120 Yanbian Yellow Cattles.C4738T mutation was founded at exon 10.significance test of meat trait means for different genetypes showed：genotype CT was negatived correlation with a＊24,b＊24 and oleic acid（P〈0.05）;and was positived correlation with tenderness,Glu,Arg,Ala,Val and Pro（P〈0.05）;genotype CC was positived correlation with Asp（P〈0.05）.