摘要
为制造疾病模型便于防治鸡肾型传支。将1日龄健康雏鸡70只分为A、B、C 3组(试验组、对照组和感染组),试验组人工接种IBV肾型毒株,感染组晚1 d放入试验组内混养,常规饲养管理,每日4次观察接毒后的临床症状,同时定期剖检病鸡和病死鸡,取多脏器固定于10%福尔马林液中、石蜡切片、HE染色,显微镜下观察组织病理学变化,按严重程度标+^++++后记录并标明曲线。结果表明,在整个试验过程中肾脏的病理变化最为严重并且持续时间较长,从第2天开始出现病变,并持续到第20天;其次是肺脏,病变出现在第5~16天;肝脏和肺脏的病变时间相同只是程度轻一些;心脏的病变出现在第6~13天。1 d后,感染组也出现相似的病理变化,说明此病的传染性很强。
Disease model was built up for control of infectious bronchitis-nephritis(IBN).Seventy 1-day-old chicks were divided into three groups named A,B and C(experimental group,control group and infected group).IBN virus was inoculated artificially to experimental group,and one day post-inoculation chicks in infected group were put into experimental group.Clinical signs were observed 4 times each day under conventional management,sick and died chicks were autopsied periodically for pathological changes observed and recorded.A few organs were sampled and fixed in 10% formalin,sectioned with paraffin and stained with Haematoxylin Eosin.Histopathological changes were observed under microscopy,recorded according to servere extention as 1 to 4 plus symbol and drawed with curved line.The results indicated that nephrotic lesions appeared the serverest and lasting longer period,from the beginning at day 2 and last to day 20.The lung,secondly,represented pathological changes during day 5 to 16.The time of changes in liver was similar to it in lung,only a little bit mild in severity.The heart showed lesions at day 6 to 13.One day later,the similar pathologic changes were appeared.This indicated that the disease was very strongly infectious.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第3期183-185,共3页
China Animal Husbandry & Veterinary Medicine
基金
天津市农业科技成果转化与推广项目<禽呼吸道病的快速诊断和综合防治技术的熟化与推广>(0701100)
关键词
鸡
肾型传支
组织病理学变化
病变曲线
chicken
infectious broncheitis-nephritis(IBN)
histopathologic changes
lesion's curve