木蝴蝶提取物制备及其抗菌抗炎活性的研究

Preparation of Oroxylum indicum Extracts and Their Anti-bacterial and Anti-inflammatory Activities

本试验对木蝴蝶提取物的制备设计了6种提取方法,分别是水提取、微波提取、60%丙酮提取及95%、70%和45%乙醇提取,采用体外抑菌试验(药敏试验和最小抑菌浓度试验)来研究木蝴蝶的抗菌活性,进行了木蝴蝶急性毒性试验和最大耐受量试验,通过二甲苯致小鼠耳廓肿胀试验来观察木蝴蝶的抗炎活性。结果表明,木蝴蝶的6种提取物对金黄色葡萄球菌和鸡大肠杆菌都有抑菌效果,其中95%乙醇的提取物对金黄色葡萄球菌和鸡大肠杆菌呈高度敏感。木蝴蝶水提取物对小鼠安全无毒,其毒性小,最大耐受量为150 g/kg体重。木蝴蝶水提取物使小鼠耳肿胀度降低,表现出较好的抗炎作用,同时木蝴蝶水提取物使小鼠胸腺指数和脾脏指数降低,表明其对小鼠免疫功能有一定的抑制作用。

Six kinds of extraction methods were used to obtain effective components from the Chinese herb,including water extraction,microwave extraction,60% acetone extraction,ethanol extraction（95%,70% and 45% ethanol）.The bacterial growth inhibition test in vitro was performed to evaluate bacteriostasis of the six kinds of extracts.The acute toxicity and maximal tolerance test on the water extract of the herb was implemented in mice.Anti-inflammatory effect of the water extract of Oroxylum indicum was observed by the ear edema induced by xylene in mice.The results showed that the six extracts of Oroxylum indicum had significant antibacterial actions on chicken Escherichia coli and Staphylococcus aureus,particularly 95% ethanol extract revealed highly sensitive to the two germs.The water extract of Oroxylum indicum was safe for animals from the findings of toxicological test and the MLD of water extract of Oroxylum indicum was 150 g/kg body weight by intragastric infuse in mice.The water extract of Oroxylum indicum had obvious anti-inflammatory effects by lowering ear swell in mice;the product also exhibited an immune suppressive action by decreasing spleen indices in mice.