沙棘酒清除自由基作用的研究

Study on the Scavenging Effects on Free Radical by Sea Buckthorn Wine

目的:研究沙棘酒对自由基的清除作用。方法:采用分光光度法测定沙棘酒对DPPH.自由基、羟基自由基和超氧阴离子自由基的清除作用,还原力的测定采用Fe3+还原法。结果:不同陈酿时间的沙棘酒清除自由基的能力不同,随着陈酿时间的延长,其清除自由基的能力呈下降趋势。沙棘酒中维生素C含量与对DPPH.自由基、羟自由基和超氧阴离子自由基的清除率之间呈正相关关系,但总黄酮含量与对上述自由基的清除率之间的相关性不显著。沙棘酒还原力与维生素C含量和总黄酮含量间均呈正相关关系。结论:沙棘酒清除DPPH.自由基、羟自由基和超氧阴离子自由基的能力较强,具有良好的抗氧化性和保健功能。

Objective： To study on the scavenging effects of free radical by sea buckthorn wine. Methods： By spec- trophotometry, scavenging effects of sea buckthorn wine on DPPH free radicals, hydroxyl free radical and superoxide anion free radical was assayed and its reducing power was assayed by Fe^3＋ reducing method. Results： The sea buckthorn wine with different natural aging time has different scavenging ability to free radical, with the natural aging time extended, the scavenging ability showed downward tendency. A positive correlation exited between vitamin C content and scavenging rate for DPPH free radicals, hydroxyl free radical, superoxide anion free radical, but the correlation exited between flavonoids content and scavenging rate for free radical became unnoticeable. A certain positive correlation exited between reducing power and vitamin C content and flavonoids content respectively. Conclusions： Sea buckthorn wine has strong ability of scavenging DPPH free radicals, hydroxyl free radical, superoxide anion free radical, which suggested that sea buckthorn wine has antioxidant activity and healthy function.