摘要
对刺葡萄皮花色苷(VSA)进行分离和检定。从刺葡萄皮花色苷水解物中得到两种花色苷元,经鉴定为锦葵啶、芍药啶;从刺葡萄皮色素中分离到5种花色苷组分,其中4种为3-,5-双糖苷,1种为3-糖苷,2种为酰化花色苷;经柱层析得到3种刺葡萄皮花色苷,经HPLC检测并计算,花色苷AN1、AN2、AN3分别占纯化样品的85.805%、1.364%及11.835%,其中AN1的纯度达99%。
The anthocyanins from Vitis skin were isolated and detected. The result showed as follows: The two kinds of anthocyandins were obtained from hydralyzate and identified as malvidin and peonidin respectively. Five kinds of anthocyanins were obtained from pigment of Vitis skin by isolation, including four kinds of 3-,5-diglycoside and one kind of 3-glycoside, and two kinds of acylatcd anthocyanins tberein. Three kinds of anthocyanins were elnted by systematic column chromatography, and AN1,AN2, AN3 were 85.805%, 1.364%, 11.835% of the anthocyanins from Vitis skin respectively through HPLC detection, and moreover, the purity of ANI was high to 99%.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2010年第1期200-206,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
湖南省自然科学基金资助项目(08JJ6005)
关键词
紫秋刺葡萄
花色苷
分离
鉴定
Vitis davidii of Ziqiu
anthocyanin
isolation
identification