摘要
目的研究骨髓间充质干细胞(BMSCs)向心肌细胞分化过程中离子通道电流的表达情况,为临床BM-SCs移植的安全性提供参考。方法分离培养BMSCs及乳鼠心肌细胞(NRVM s),并以1∶10的比例共培养,应用全细胞膜片钳技术记录共培养的BMSCs上离子通道电流的表达情况。结果成功分离培养BMSCs和NRVM s并建立了共培养模型,共培养1周后,BMSCs形态发生了明显变化;在心肌微环境诱导下,向心肌分化的BMSCs平均膜电位为-(40.37±3.68)mV,平均膜电容为(35.18±4.96)pF;共培养的BMSCs上可记录到IK1、Ito、IKsus,未记录到INa和ICa,也未记录到KCa。结论BMSCs和NRVM s共培养1周后,BMSCs上记录到IK1、Ito及IKsus,但未记录到INa和ICa。
Objective To observe the membrane currents recorded in bone mesenchymal stem cells(BMSCs) differentiating into cardiomyocytes and to provide the reference for the safety and utility of BMSC transplantation.Methods BMSCs and neonatal rat ventricular myocytes(NRVMs) were isolated respectively,and colcultured at a ratio of 1∶10.The whole-cell patch clamp technique was used to record ionic currents in BMSCs after colcultured with cardiomyocytes.Results BMSCs and NRVMs were successfully isolated and cultivated.Then the cocultured model was founded.The average cellular membrane potential of differentiated BMSCs was about-(40.37±3.68)mV,and the mean capacity was about(35.18±4.96)pF.After 1 week coculture,the voltage-clamp experiment showed that IK1、Ito and IKsus were recorded in differentiated BMSCs,but KCa disappeared.Additionally,INa and ICa were still not recorded in differentiated BMSCs.Conclusion IK1、Ito and IKsus,but not INa and ICa may be recorded in BMSCs differentiating into cardiomyocytes induced by cardiac microenvironment.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2010年第1期26-28,32,共4页
Journal of Harbin Medical University
基金
黑龙江省卫生厅课题(2007-476)
黑龙江省教育厅科学技术研究课题(11541163)
黑龙江省博士后基金资助项目
关键词
骨髓间充质干细胞
心肌细胞
膜片钳
膜电流
bone mesenchymal stem cells
cardiomyocyte
patch clamp
membrane current