清热滋阴活血复方制剂对大鼠糖尿病牙周炎的疗效及外周血TNF-α的影响

Effect of Qingreziyinhuoxue recipe on diabetic periodontitis and peripheral blood TNF-α of rats

目的:通过动物实验,观察清热滋阴活血中药复方制剂对大鼠糖尿病牙周炎的疗效,并初步探讨其机制。方法:10周龄健康雄性Wistar大鼠随机分为健康对照组(A组)、糖尿病牙周炎组(B组)和中药治疗组(C组),B组与C组糖尿病牙周炎造模成功后,C组灌胃等效剂量的金银花、北沙参、川芎复方水煎剂,A组与B组灌胃等体积的生理盐水,2个月后,麻醉下方块截取包括实验区磨牙的双侧上颌骨,HE染色后,选择包括牙龈结缔组织及上皮组织的视野计数浸润的炎性细胞,测量实验牙近中牙槽嵴顶至釉牙骨质界的距离;腹腔总静脉抽取静脉血,制备血清后用ELISA方法检测TNF-α的含量,应用SPSS10.0软件对数据进行单因素方差分析。结果:B组与C组牙龈组织的浸润炎性细胞数目、牙槽嵴顶至釉牙骨质界的距离较A组均有增高,且B组高于C组,三组两两比较差异均具有统计学意义(P<0.05);B组外周血TNF-α的含量较A组与C组高,差异均有统计学意义(P<0.05),但A组与C组间无统计学差异(P>0.05)。结论:清热滋阴活血中药复方制剂对糖尿病牙周炎有治疗作用,可降低外周血TNF-α的水平。

Objective：To evaluate the e ffect of Qingreziyinhuoxue recipe on diabetic periodontitis and peripheral blood TNF-α of rats. Method：10 weeks old Wistar rats were randomly divided into control group （group A）,model group （group B）and Chinese herbal group（group C）. The diabetic periodontitis model were made in groups B and C. After being gavaged with Chinese herb/normal saline for 2 months,the level of TNF-α in peripheral blood was detected by enzymelinked immunosorbent assay（ELISA）,periodontal inflammation was quantified by the inflammatory cell infiltration in gingival regions,whereas periodontal destruction was assessed by the alveolar bone loss in the experimental regions. All the results were analyzed by SPSS 10.0 software package. Result：The amount of inflammatory cell infiltration and the alveolar bone loss were both higher in groups B and C than in group A. The differences were found to be significant between groups A-B,groups B-C,and groups A-C. The level of TNF-α in group B was higher than groups A and C. There were statistically significant differences between groups B-A and groups B-C,but not between groups A-C. Conclusion：Qingreziyinhuoxue recipe has therapeutic effect on diabetic periodontitis in rats. It can lower the level of TNF-α in peripheral blood.