四川省小麦条锈菌群体遗传多样性的SSR分析

SSR analysis of population genetic diversity of Puccinia striiformis f.sp.tritici in Sichuan Province,China

利用TP-M13-SSR自动荧光检测技术,对四川省小麦条锈菌群体遗传多样性水平进行了分析。研究结果表明,四川省小麦条锈菌群体遗传多样性比较丰富,地区之间存在明显的差异,川西北和四川盆地的种群遗传多样性相对较高,而四川西南部和四川东南部的种群遗传多样性较低。四川小麦条锈菌群体存在一定的遗传分化,地区间的遗传变异仅占14.92%,群体间的遗传变异占总变异的23.06%,群体内遗传变异占60.02%,遗传变异主要存在于群体内部。基因流和共享基因型从分子水平证实了四川小麦条锈菌在地区间的传播,且川西北和四川盆地之间的菌源交流最为广泛。

Stripe rust（or yellow rust） of wheat（Triticum aestivum）,caused by Puccinia striiformis f.sp.tritici,is one of the most important diseases of wheat in China.Since Sichuan is one of the most important over-summering areas of P.striiformis f.sp.tritici in China,the objective of this study was to determine genetic structure of P.striiformis f.sp.tritici populations in this region.Population genetic diversity was investigated for the P.striiformis f.sp.tritici population from different areas in Sichuan province with TP-M13-SSR technique.The important conclusion is that P.striiformis f.sp.tritici populations were considerably diversity in Sichuan,while there were different between regions.Genetic diversity for the pathogen population from northwest of Sichuan and Sichuan Basin were much higher than that from other regions.Genetic differentiation was also investigated.The results showed that interregional genetic variation accounted for 14.92% of the total,while interpopulational variation 23.06% and intrapopulational variation 60.02%.Moreover,our molecular data on gene flow and genotype confirmed the migration of pathogen populations among regions in Sichuan,in particular,between northwest of Sichuan and Sichuan Basin.