摘要
Suaeda salsa L. is a halophytic species that is well adapted to high salinity. In order to understand its salt tolerance mechanism, we examined the growth and vacuolar H^＋-ATPase （V-ATPase） response to NaCI within the shoots and roots. The growth of shoots, but not roots, was dramatically stimulated by NaCI. CI^- and Na^＋ were mainly accumulated in shoots. V-ATPase activity was significantly increased by NaCI in roots and especially in shoots. Interestingly, antisera ATP95 and ATP88b detected three V1 subunits （66, 55 and 36 KDa） of V-ATPase only in shoots, while an 18 kDa V0 subunit of V-ATPase was detected by both antisera in shoots and roots. It suggested that the tissue-specific characteristics of V-ATPase were related to the different patterns of growth and ion accumulation in shoots and roots of S. salsa.
Suaeda salsa L. is a halophytic species that is well adapted to high salinity. In order to understand its salt tolerance mechanism, we examined the growth and vacuolar H^＋-ATPase （V-ATPase） response to NaCI within the shoots and roots. The growth of shoots, but not roots, was dramatically stimulated by NaCI. CI^- and Na^＋ were mainly accumulated in shoots. V-ATPase activity was significantly increased by NaCI in roots and especially in shoots. Interestingly, antisera ATP95 and ATP88b detected three V1 subunits （66, 55 and 36 KDa） of V-ATPase only in shoots, while an 18 kDa V0 subunit of V-ATPase was detected by both antisera in shoots and roots. It suggested that the tissue-specific characteristics of V-ATPase were related to the different patterns of growth and ion accumulation in shoots and roots of S. salsa.
基金
Supported by the National NaturalScience Research Foundation of China (30670177)
the Hi-Tech Research and Development (863) Program of China(2007AA091701)
Specialized Research Fund for the Doc-toral Program of Higher Education (20050445003)
