新疆哈萨克族冠心病患者eNOS基因G894T多态性分析

Study on polymorphism of eNOS gene G894T in Kazak patients with CHD in Xinjiang

目的探讨新疆哈萨克族人群内皮型一氧化氮合酶(eNOS)基因多态性与冠心病的相关性。方法应用聚合酶链反应(PCR)-限制性片段长度多态性分析(PCR-RFLP),检测新疆哈萨克族70例健康个体和87例冠心病患者eNOS基因G894T多态性。结果新疆哈萨克族健康个体及冠心病患者的eNOS基因G894T多态性GG、GT、TT基因型频率分布分别为51.43%、30.00%、18.57%和24.14%、55.17%、21.69%,G和T等位基因分布频率分别为75.71%、24.29%和56.32%、43.68%,冠心病患者存在G等位基因频率下降,T等位基因频率升高趋势。结论eNOS基因G894T多态性分析,与新疆哈萨克族冠心病有相关性。

Objective To study the relationship between coronary heart disease（CHD） and polymorphism of gene of nitric oxide synthase （ eNOS G894T） in Kazaks in Xinjiang. Methods The eNOS genc related polymorphism of G894T was detected by polymerized chain reaction - restriction fr^Tnent length polymorphism （ PCR - RFLP） in 70 cases of CHD and 87 healthy subjects. Results Result of tests showed that fre- quencies of GG, GT and TY of eNOS gene were 51.43% , 30.00%, 18.57% and 24.14% , 55.17%, 21.69% in healthy group and CHD group respectively. The allele frequencies of G and T were 75.71%, 24.29% and 56.32%, 43.68% respectively. Allele frequency of G was descen- ded in CHD patients and allele frequency of T had been raised in CHD patients. Conclusions It can be concluded as that the allele frequency of polymorphism of G894T of eNOS gcne is correlated with CHD patients in Kazaks in Xinjiang.