3-脱氧葡萄糖醛酮对正常小鼠血糖的影响

Effects of 3-deoxyglucosone on blood glucose of normal mice

目的观察外源性3-脱氧葡萄糖醛酮(3-DG)对正常小鼠血糖的影响。方法雄性昆明种小鼠12只,随机分为3-DG组(5mg/kg)和对照组(Con组),各6只。灌胃3-DG第1天、1周、2周后分别采用血糖仪测定小鼠自由饮食和空腹状态下的血糖;并对灌胃3-DG2周的小鼠进行糖耐量实验。结果(1)小鼠单次灌服3-DG后2h的血糖比灌胃前升高(7.78±0.694 vs 6.41±0.408,P<0.05),与Con组比显著升高(7.78±0.694vs 6.43±0.427,P<0.01);连续灌服3-DG 1周,3-DG组多时间点的血糖均比Con组升高,但差异无统计学意义(P>0.05)。(2)小鼠单次灌服3-DG后空腹6h与连续灌服3-DG 2周后空腹血糖与Con组比,均无统计学差异(P>0.05)。(3)小鼠连续灌服3-DG 2周,与Con组比,糖耐量30min(19.47±1.541 vs 17.22±1.911,P<0.05)、60min血糖(P>0.05)升高,120min血糖无变化(P>0.05)。结论外源性3-DG能够升高正常小鼠血糖水平,主要表现在单次灌胃3-DG对小鼠血糖有即时升高的作用和连续灌胃引起血糖生理曲线上移及糖耐量轻度受损。

Objective To study the effect of exogenous 3-deoxyglucosone （3-DG） on blood glucose of normal mice. Methods Kunming male mice were divided into two groups： 3-DG （5mg/kg） treatment and control group. At day 1, week 1 and week 2 after treatment, we compared fasting blood glucose, and did the glucose tolerance test in 3-DG group after 2 weeks＇ 3-DG administration. Results Blood glucose was increased obviously 2h after administration with 3-DG once versus pre-administration and control （7. 78 ± 0. 694 vs 6.41 ±0. 408,and 6. 43±0. 43,all P〈0. 05）. After 1 week, 3-DG group＇s blood glucose at different time points were higher than control,but no statistical significance （P〈0.05）. There were no differences in 6 hour fasting blood glucose after once administration with 3-DG and in 12 hours fasting blood glucose two weeks after administration with 3-DG between 3-DG and Con groups. After 3-DG administration for two weeks,as compared with Con, the 30 minute blood glucose of 3-DG; administration mice during OGTT was obviously increased （19.47±1. 541 vs 17. 22±1. 911,P〈0. 05）, but glucose levels at 60 minute and 120 minute had no changes （P〉0. 05）. Conclusions Exogenous 3-DG increases the normal mice＇s blood glucose immediately after once administration with 3-DGand results in up-moving of glucose physiological curve and leads to a light impairment of glucose tolerance when mice were continuously fed with 3-DG for 2 weeks.