桑树组培叶片不定芽诱变研究

STUDIES ON INDUCED VARIATION OF ADVENTITIOUS BUDS OF MULBERRY LEAVES IN TISSUE CULTURE

对组培桑树叶片上的不定芽原体进行了Ｃｏ６０γ射线辐照，秋水仙素、甲基磺酸乙酯处理；观察了诱变因素对叶片不定芽生长分化的影响及变异情况。Ｃｏ６０γ射线辐照，以１５００Ｒ剂量处理变异率最高，为２０３１％，ＬＤ５０值为１５５０Ｒ；秋水仙素处理，以浓度０６％变异率较高为３８２６％，ＬＤ５０值为０５４％（处理１ｄ）。并对变异植株进行了大田移栽。

The proembryos of adventitious buds of mulberry leaves in tissue culture were treated with radiation of Co 60 γ rays,colchicine and Ethy1 methylsulfonate respectively.Effects of mutagenic factors on growing and differentiation of adventitious buds of leaves and variatons were observed.Of Co 60 γ rays,variation of 1 500R was 20.31%,the highest,and its LD 50 was 1 550R.While of concentration of colchicine,variation of 0.6% was 38.26%,the highest,and its LD 50 was 0.54%.The variant plants were transplanted in the field.